Structural basis for binding of cyclic 2-oxoglutarate analogues to factor-inhibiting hypoxia-inducible factor

Bioorg Med Chem Lett. 2010 Oct 15;20(20):6125-8. doi: 10.1016/j.bmcl.2010.08.032. Epub 2010 Aug 10.

Abstract

Aromatic analogues of the 2-oxoglutarate co-substrate of the hypoxia-inducible factor hydroxylases are shown to bind at the active site iron: Pyridine-2,4-dicarboxylate binds as anticipated with a single molecule chelating the iron in a bidentate manner. The binding mode of a hydroxamic acid analogue, at least in the crystalline state, is unusual because two molecules of the inhibitor are observed at the active site and partial displacement of the iron binding aspartyl residue was observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Catalytic Domain
  • Humans
  • Ketoglutaric Acids / chemistry*
  • Ketoglutaric Acids / pharmacology*
  • Mixed Function Oxygenases
  • Models, Molecular
  • Protein Binding
  • Repressor Proteins / chemistry
  • Repressor Proteins / metabolism*

Substances

  • Ketoglutaric Acids
  • Repressor Proteins
  • Mixed Function Oxygenases
  • HIF1AN protein, human